Open AccessStability and activity of hydrogenases of Methanobacterium thermoautotrophicum and Alcaligenes eutrophus in reversed micellar systems
Author(s) -
Hoppert Michael,
Braks Iris,
Mayer Frank
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06836.x
Subject(s) - hydrogenase , methanobacterium , alcaligenes , aqueous solution , microemulsion , chemistry , bacteria , nuclear chemistry , hydrogen , inorganic chemistry , biochemistry , organic chemistry , biology , pulmonary surfactant , pseudomonas , genetics , archaea , gene
In water‐in‐oil microemulsion the membrane‐associated F 420 ‐hydrogenase of Methanobacterium thermoautotrophicum (strain Marburg) and the membrane‐bound hydrogenase of Alcaligenes eutrophus H 16 (MBH) showed prolonged activity at elevated temperatures (measured as hydrogen production) as compared to aqueous buffer solution. The temperature optimum of the reactions was about 15°C higher than in aqueous buffer solution. Activity of the almost completely inactivated F 420 ‐hydrogenase could be partially recovered by transfer into microemulsion.