Open AccessSalmonella typhimurium enterotoxin epitopes shared among bacteria
Author(s) -
Chopra Ashok K.,
Xu XinJing,
Peterson Johnny W.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06834.x
Subject(s) - salmonella , enterotoxin , microbiology and biotechnology , epitope , bacteria , enterobacteriaceae , biology , escherichia coli , antibody , genetics , gene
The deduced amino acid sequence of the cloned Salmonella enterotoxin gene ( stn ) was used to prepare anti‐peptide antibodies. These antibodies were then employed to screen isolates of this enteric pathogen for the synthesis of protein enterotoxin (Stn). Cell lysates of all Salmonella isolates tested displayed a prominent immunoblot band of approximately 29 kDa, which was consistent with the size of the cloned stn gene product. Among other Gram‐negative bacteria examined, isolates of Klebsiella Enterobacter , and Citrobacter exhibited a similar‐sized protein that reacted strongly with the Stn antibodies. Since the stn gene was located opposite the hydrogenase regulatory genes ( hydHG ) required for hydrogen metabolism in bacteria, our data suggested that only in Salmonella and some other members of the family Enterobacteriaceae had the DNA sequence evolved, presumably through point mutations, into an expressed gene product of similar size.