Open AccessComparative development of Cryptosporidium parvum (Apicomplexa) in 11 continuous host cell lines
Author(s) -
Upton Steve J.,
Tilley Michael,
Brillhart Dianne B.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06833.x
Subject(s) - cryptosporidium parvum , parasite hosting , apicomplexa , biology , cell culture , cryptosporidium , host (biology) , incubation , incubation period , virology , microbiology and biotechnology , immunology , protozoal disease , genetics , feces , biochemistry , world wide web , computer science , malaria
Using standardized media, incubation, and parasite inoculating procedures, we compared development of Crytosporidium parvum between Madin‐Darby bovine kidney (MDBK) cells and 10 additional host cell lines available through the American Type Culture Collection. Parasite development was assessed by counting parasite numbers atop monlayers in 25 random oil fields 68 h post‐infection using Nomarski interference‐contrast optics. Results revealed that the human ileocecal adenocarcinoma (HCT‐8) cell line supported nearly twice the number of parasite developmental stages than MDBK cells or any of the other host cell types.