Open AccessIdentification of the rpmF‐plsX‐fabH genes of Rhodobacter capsulatus
Author(s) -
Carty Sherry M.,
Colbeau Annette,
Vignais Paulette M.,
Larson Timothy J.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06832.x
Subject(s) - rhodobacter , escherichia coli , biology , gene cluster , gene , biochemistry , gene product , homology (biology) , atp synthase , microbiology and biotechnology , mutant , gene expression
The rpmF‐plsX‐fabH gene cluster of Rhodobacter capsulatus homologous to that of Escherichia coli was identified. rpmF encodes ribosomal protein L32, plsX plays an undefined role in membrane lipid synthesis, and fabH encodes β‐ketoacyl‐acyl carrier protein synthase III. The R. capsulatus plsX gene complemented a defect in an E. coli strain with the plsX50 mutation. Overproduction of the fabH gene product of R. capsulatus in E. coli resulted in dramatically increased β‐ketoacyl‐acyl carrier protein synthase III activity. These results indicate that plsX and fabH apparently function the same in R. capsulatus as in E. coli .