Open AccessSelection and study of a Candida molischiana mutant derepressed for β‐glucosidase production
Author(s) -
Janbon G.,
Arnaud A.,
Galzy P.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06829.x
Subject(s) - mutant , selection (genetic algorithm) , biology , production (economics) , microbiology and biotechnology , genetics , gene , computer science , artificial intelligence , macroeconomics , economics
A mutant strain of Candida molischiana was selected. Analysis of the exocellular activity of Candida molischiana 35M5N grown on different carbon sources revealed that the biosynthesis of β‐glucosidase is derepressed in this yeast strain. The strain is not a hyper‐producer mutant. There were no observed differences in the endocellular and parietal activities of the wild and mutant strains. However, the mutant strain produced 35‐fold more enzyme than the wild‐type in the culture medium with glucose as carbon source. When glucose was used as carbon source, the mutant strain produced 90% more exocellular enzyme than when cellobiose was used as the carbon source.