Open AccessIdentification of the siderophores from Vibrio hollisae and Vibrio mimicus as aerobactin
Author(s) -
Okujo Noriyuki,
Yamamoto Shigeo
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06824.x
Subject(s) - aerobactin , siderophore , molecular mass , microbiology and biotechnology , vibrionaceae , bacterial outer membrane , enterobacteriaceae , biology , vibrio , bacteria , chemistry , mass spectrometry , biochemistry , escherichia coli , chromatography , gene , genetics , enzyme
Hydroxamate siderophores were purified from low‐iron cultures of Vibrio hollisae ATCC 33564 and Vibrio mimicus ATCC 33653. By a combination of 1 H and 13 C NMR spectroscopy, fast atom bombardment mass spectrometry, and compositional analysis, both of the siderophores were identified as aerobactin, a citrate‐based dihydroxamate siderophore, which is highly prevalent in species of the family Enterobacteriaceae . Four other clinical strains belonging to these species also produced aerobactin. In response to iron limitation, all strains expressed two high molecular mass outer membrane proteins. The protein with an apparent molecular mass of 77 kDa, which was common to all strains examined, may be the ferric aerobactin receptor.