Open AccessHaemagglutination activity of toxigenic and non‐toxigenic strains of Clostridium difficile
Author(s) -
Meng X.Q.,
Yamakawa K.,
Ogura H.,
Nakamura S.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06816.x
Subject(s) - antiserum , microbiology and biotechnology , hemagglutination , clostridium difficile , strain (injury) , toxin , clostridium botulinum , clostridium , ammonium sulfate precipitation , column chromatography , biology , chemistry , chromatography , bacteria , biochemistry , virology , antibody , virus , size exclusion chromatography , anatomy , immunology , enzyme , antibiotics , genetics
Cell extract of Clostridium difficile strains was fractionated by ammonium sulfate precipitation and sulfated cellulofine column chromatography to detect haemagglutination (HA) activity. HA activity without cytotoxicity was detected in fractions eluted at 0.79–0.91 M NaCl in sulfated cellulofine column chromatography of the cell extract in both toxigenic strain VPI 10463 and non‐toxigenic strain KZ 1678, while toxin A was detected in fractions eluted at 0.27–0.29 M NaCl. Antisera were prepared with HA substance‐containing fractions of the chromatography. Antiserum to the HA substances(s) of strain VPI 10463 neutralised the HA activity of the fractions of strains VPI 10463 and KZ 1678 at nearly the same titres. Antiserum to the HA substance(s) of strain KZ 1678 also neutralised the HA activity of both strains at nearly the same titres as above. These findings suggest that haemagglutinin(s) is commonly produced by C. difficile strains irrespective of toxin A‐producing ability.