Open AccessCitrate transport in Lactococcus lactis biovar diacetylactis: Expression of the plasmid‐borne citrate permease P
Author(s) -
Magni Christian,
Felipe Felix López,
Sesma Fernando,
López Paloma,
Mendoza Diego
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06806.x
Subject(s) - biovar , lactococcus lactis , permease , plasmid , subcloning , biology , microbiology and biotechnology , biochemistry , gene , chemistry , bacteria , transporter , genetics , lactic acid
The Lactococcus lactis subsp. lactis biovar diacetylactis citrate permease P is encoded by the citP gene borne by an 8.3‐kb Cit + plasmid (F. Sesma, Gardiol, D., de Ruiz Holgado, A.P. and de Mendoza, D., Appl. Environ. Microbiol. 56 (1990) 2099–2103). By subcloning different DNA fragments from the Cit + plasmid it was found that citP is the only plasmidic gene required for the transport of citrate. Northern blot analysis revealed that citP is transcribed in two mRNA species with lengths of 2.9 and 1.9 kb. The transcriptional pattern, the total amount of citP mRNAs and the transport of citrate were unaffected by the presence of citrate in the growth media. Our results support the conclusion that transcription of citP as well as citrate transport are not induced by citrate in Lactococcus lactis subsp. lactis biovar diacetylactis.