Open AccessPhylogeny of human intestinal spirochaetes inferred from 16S rDNA sequence comparisons
Author(s) -
Hookey J.V.,
Barrett S.P.,
Reed C.S.,
Barber P.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06790.x
Subject(s) - spirochaete , biology , phylogenetic tree , phylogenetics , genetics , 16s ribosomal rna , sequence (biology) , sequence analysis , lineage (genetic) , microbiology and biotechnology , dna , bacteria , gene
The sequence of 1383 nucleotides of the DNA encoding 16S rDNA was determined for strains of human intestinal spirochaetes, comprising an unnamed isolate and “ Brachyspira aalborgi ” NCTC 11492. A phylogenetic tree was inferred from aligned sequence comparisons between the intestinal spirochaetes, representatives of the Spirochaetales and Escherichia coli . The type strain of Brachyspira aalborgi , though related to the Serpulina spp. at approx. 96.5% sequence similarity was distinct and separated from the unnamed human intestinal isolate, HIS Oman, N26. The latter formed a separated and novel lineage that bisected the Spirochaetales.