Open AccessEscherichia coli ferric uptake regulator (Fur) can mediate regulation of a pseudomonad iron‐regulated promoter
Author(s) -
O'Sullivan Daniel J.,
Dowling David N.,
DeLorenzo Victor,
O'Gara Fergal
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06787.x
Subject(s) - escherichia coli , promoter , biology , microbiology and biotechnology , mutant , pseudomonas , lac operon , heterologous , dna footprinting , transcriptional regulation , gene , transcription factor , gene expression , biochemistry , genetics , bacteria
of a Pseudomonas iron‐regulated promoter lacZ fusion (SP1) and a Pseudomonas transcriptional factor into Escherichia coli allowed expression of the promoter in this heterologous host. Evaluation of this promoter in wild‐type and fur mutants of E. coli , by measuring β‐galactosidase activity, indicated that E. coli Fur can regulate the Pseudomonas promoter in response to iron starvation. Gel retardation assays suggested that purified Fur protein could interact with the SP1 promoter upstream of the transcriptional start. DNase I footprinting analysis established that Fur protected a primary 58‐bp region (−50 to −106 bp). These protein/DNA interactions correlate with the observed in vivo regulation of the SP1 promoter in E. coli and indicate that Fur can functionally regulate a Pseudomonas iron‐regulated promoter.