Open AccessUDP‐ N ‐acetylglucosamine 1‐carboxyvinyl‐transferase from Acinetobacter calcoaceticus
Author(s) -
Ehrt Sabine,
Hillen Wolfgang
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06755.x
Subject(s) - acinetobacter calcoaceticus , rpon , biology , genetics , open reading frame , plasmid , bacteria , gene , acinetobacter , peptide sequence , promoter , gene expression
We have analyzed the sequence downstream of rpoN from Zcinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to UDP‐ N ‐acetylgucosamine 1‐carboxyvinyl‐transferase (MurZ). Multicopy plasmids encoding this enzyme conferred phosphomycin resistance to A. calcoaceticus . The polar effect of a rpoN mutation on the phosphomycin resistance level suggests that murZ is, in part, cotranscribed with rpoN . These observations confirm that A. calcoaceticus represents the first exceptin from a conserved genetic context of rpoN observed in several other Gram‐negative bacteria.