Open AccessCharacterization of restriction endonuclease Ajo I from Acinetobacter johnsonii
Author(s) -
Nowak A.,
Kur J.,
Gospodarek E.,
Bielawski K.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06748.x
Subject(s) - psti , isoschizomer , restriction enzyme , biology , microbiology and biotechnology , genetics , dna , biochemistry , gene , gene expression , dna methylation
A new type II restriction endonuclease, named Ajo I, was detected in Acinetobacter johnsonii . The enzyme Ajo I, an isoschizomer of Pst I, recognized the hexanucleotide sequence [5′‐CTGCA/G‐3′], with a cleavage site generating fragments of DNA with protruding cohesive 3′ termini.