Open AccessSerotyping Coxiella burnetii isolates from acute and chronic Q fever patients by using monoclonal antibodies
Author(s) -
Yu Xuejie,
Raoult Didier
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06735.x
Subject(s) - coxiella burnetii , q fever , endocarditis , virology , monoclonal antibody , serotype , rickettsiosis , microbiology and biotechnology , rickettsiales , antibody , biology , medicine , immunology , rickettsia , bacteria , virus , genetics
Four mouse monoclonal antibodies reacting with Coxiella burnetii lipopolysaccharide antigens were produced and used in serotyping 17 C. burnetii isolates from acute Q fever and Q fever endocarditis patients in France. Two monoclonal antibodies (1B2 and 3B6) were considered specific for the Priscilla strain, a representative of Q fever endocarditis isolates, and did not react with the Nine Mile strain, which is representative of acute Q fever isolates. Monoclonal antibodies Nos. 1B2 and 3B6 reacted with 75% (3/4) acute Q fever isolates and 85% (11/13) of endocarditis isolates from France. It is reasonable to conclude that Priscilla‐like strains cause both acute Q fever and Q fever endocarditis. The hypothesis that Priscilla‐like strains only are associated with Q fever endocarditis should be reconsidered.