Open AccessPectolytic enzymes produced by Pseudomonas syringae pv. glycinea
Author(s) -
Magro Paolo,
Varvaro Leonardo,
Chilosi Gabriele,
Avanzo Cristina,
Balestra Giorgio Mariano
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06733.x
Subject(s) - pseudomonas syringae , pectinase , enzyme , pectate lyase , pseudomonas , strain (injury) , pseudomonadaceae , chemistry , pseudomonadales , microbiology and biotechnology , bacteria , biochemistry , biology , genetics , anatomy , gene
Six strains of Pseudomonas syringae pv. glycinea secreted, in vitro, the pectolytic enzymes pectate lyase (PL) and polygalacturonase (PG). All six bacterial strains were capable of producing both enzymes, but at different levels. Strain NCPPB 2411 secreted a large amount of PG and the highest level of PL. Both enzymes exhibited temperature optima between 30 and 40°C. PL exhibited a pH optimum at 8.0, while the pH optimum of PG was 5.0. For maximum activity PL required Ca 2+ . Upon isoelectrofocusing (IEF), the culture supernatant of strain NCPBB 2411 exhibited 2 alkaline PLs (pI 9.0, 9.5) and one alkaline PG (pI 8.5). It is possible that the pectolytic enzymes are involved in the infection process.