Open AccessMitogenic stimulation of murine B lymphocytes by the N‐acetyl‐D‐galactosamine specific bacterial lectin‐like substance from Eikenella corrodens
Author(s) -
Nakae Hideaki,
Yumoto Hiromiti,
Matsuo Takashi,
Ebisu Shigeyuki
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06726.x
Subject(s) - lectin , stimulation , galactosamine , chemistry , microbiology and biotechnology , biology , biochemistry , endocrinology , glucosamine
A N‐acetyl‐D‐galactosamine (GalNAc) specific bacterial lectin‐like substance from Eikenella corrodens 1073 (EcLS) was found to have potent mitogenic activity when cultured with splenocytes from BALB/c mice. The results indicated that B lymphocytes are the major cell type responding to EcLS. The mitogenic activity of EcLS was dose‐dependent, and the optimal concentration was around 5 μg/ml. The mitogenic activity did not appear to be due to a bacterial endotoxin, as GalNAc inhibited the mitogenic activity of EcLS, but did not inhibit the activity of lipopolysaccharide isolated from E. corrodens . EcLS stimulated murine B lymphocytes not only to proliferate, but also to differentiate into antibody‐secreting cells, as demonstrated by the production of immunoglobulin by B lymphocytes stimulated with EcLS. These findings suggest that EcLS is a novel lectin that not only induces B lymphocyte proliferation, but also differentiation.