Open AccessExpression of an heterologous gene activating actinorhodin biosynthesis in Streptomyces lividans and Streptomyces coelicolor
Author(s) -
Romero Natalia M.,
Parro Víctor,
Mellado Rafael P.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06719.x
Subject(s) - actinorhodin , streptomyces coelicolor , heterologous expression , heterologous , biosynthesis , gene , chemistry , biology , biochemistry , microbiology and biotechnology , recombinant dna , mutant
Production of the blue‐pigmented antibiotic actinorhodin resulted in activation in the non‐producer strain Streptomyces lividans , but not in the natural producer strain Streptomyces coelicolor , when transformed with an heterologous activator gene from Streptomyces fradiae . The gene encodes a 132 nucleotide‐long transcript, responsible for the actinorhodin production phenotype, and thought to act as a putative antisense RNA, which has been detected in the transformed S.lividans cultures by reverse transcription followed by cyclic amplication.