Open AccessRestriction fragment length polymorphisms of 16S rRNA genes in the differentiation of fast‐growing mycobacterial species
Author(s) -
Domenech P.,
Menendez M.C.,
Garcia M.J.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06669.x
Subject(s) - restriction fragment length polymorphism , biology , terminal restriction fragment length polymorphism , 16s ribosomal rna , genetics , gene , restriction fragment , dna , mycobacterium fortuitum , ribosomal rna , mycobacterium , microbiology and biotechnology , bacteria , genotype
DNA from several species of fast growing mycobacteria displayed a characteristic restriction fragment length polymorphism (RFLP) pattern when hybridizated to a Mycobacterium fortuitum 16S rRNA gene fragment. The resulting patterns were identical when comparing different strains belonging to the same species. The RFLP results were consistent with those obtained by DNA‐DNA hybridization studies. Using this approach, we have been able to identify the number of copies for 16S rRNA genes in several fast‐growing mycobacteria.