Open AccessPhylogenetic analysis of Kineococcus aurantiacus based on 16S rRNA gene sequences
Author(s) -
Takeuchi Mariko,
Yokota Akira
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06667.x
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , actinobacteria , genetics , gene , ribosomal rna , sanger sequencing , sequence analysis , phylogenetics , polymerase chain reaction , dna sequencing
A 16S rRNA gene (rDNA) of Kineococcus aurantiacus was amplified by polymerase chain reaction (PCR) from bacterial cells and subjected to direct sequencing by the Sanger dideoxynucleotide chain termination method. The primary structure was compared with those of 32 representative actinomycetes and related organisms. The phylogenetic analysis indicated that K. aurantiacus is a member of the actinobacteria, but it shows no close relationship to any member of the order Actinomycetales, for which 16S rRNA sequences are available. Thus, this genus seems to be unique and forms a novel line of descent among the previously described Gram‐positive cocci and rods.