Open AccessCoinduction of α‐L‐arabinofuranosidase and α‐D‐galactosidase formation in Trichoderma reesei RUT C‐30
Author(s) -
Kristufek Doris,
Hodits Regina,
Kubicek Christian P.
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06648.x
Subject(s) - trichoderma reesei , chemistry , microbiology and biotechnology , biochemistry , biology , enzyme , cellulase
Formation of α‐L‐arabinosidase can be induced in Trichoderma reesei by growing the fungus on L‐arabinose or dulcitol, and by adding L‐arabinose, L‐arabitol, D‐galactose, or dulcitol ot non‐growing mycelia. The same conditions also stimulated the formation of α‐D‐galactosidase, but not that of various other enzymes involved in hemicellulose degradation. The optimal inducer concentration with all compounds was 4 mM for both enzymes. Using L‐arabinose and D‐galactose, the induction efficiency was highest at pH 6.5, whereas induction by arabitol and dulcitol was more efficient at low pH (2.5). The addition of 50 mM glucose did not repress α‐L‐arabinosidase or α‐D‐galactosidase formation. These findings suggest coregulation of two hemicellulose side‐chain cleaving enzymes in T. reesei .