Open AccessPurification and characterization of Vibrio cholerae O139 fimbriae
Author(s) -
Yamashiro Tetsu,
Nakasone Noboru,
Honma Yasuko,
John Albert M.,
Iwanaga Masaaki
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06646.x
Subject(s) - fimbria , vibrio cholerae , pilus , microbiology and biotechnology , vibrionaceae , cholera , bacteria , biology , protein subunit , strain (injury) , enterobacteriaceae , escherichia coli , biochemistry , genetics , gene , anatomy
A Vibrio cholerae O139 (strain Al‐1841) isolated from a patient with a cholera‐like disease in Bangladesh predominantly produced new curved, wavy fimbriae (Al‐1841 fimbriae) and small numbers of previously reported V. cholerae non‐O1 S7‐like pili. The former was purified and characterized. The molecular mass of the Al‐1841 fimbrial subunit was less than 2.5 kDa, and it was immunologically different from that of V. cholerae non‐O1 S7 pili. This novel fimbrial antigen was detected in all 182 Gram‐negative strains from five genera tested but was absent from the Gram‐positive bacteria tested. The purified Al‐1841 fimbriae did not agglutinate human or rabbit erythrocytes.