Open AccessPhospholipid requirements of membrane‐bound chitin synthase from Absidia glauca
Author(s) -
Machida Sachiko,
Todoriki Setsuko,
Hamamatsu Shioka,
Saito Michihiko
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06644.x
Subject(s) - phospholipid , phosphatidylcholine , chitin , biochemistry , enzyme , phosphatidylinositol , enzyme assay , chemistry , chitin synthase , glycolipid , biosynthesis , biology , membrane , signal transduction , chitosan
Membrane‐bound chitin synthase, a key enzyme in chitin biosynthesis, had a specific requirement for phospholipid. The activity of the enzyme was enhanced 2.7‐fold by adding phosphatidylinositol from porcine liver but not by other phospholipids. Each of the constituents of phospholipids inhibited enzyme activity at concentrations over 0.05%. Sterols and glycolipids had little effect on chitin synthase activation. Moreover, investigation using define species of phosphatidylcholine revealed that 1‐palmytoyl‐2‐arachidoyl and 1‐stearoyl‐2‐arachidoyl phosphatidylcholine activated the enzyme. In contrast to the arachidoyl acyl chain, other species having unsaturated fatty acyl chains inhibited enzyme activity at a concentration of 0.01%.