Open AccessPurification and characterization of distinct type of mannose‐sensitive fimbriae from Salmonella typhimurium
Author(s) -
Ghosh Sujata,
Mittal Amrisha,
Ganguly Nirmal Kumar
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06643.x
Subject(s) - fimbria , mannose , protein subunit , salmonella , gel electrophoresis , microbiology and biotechnology , strain (injury) , enterobacteriaceae , polyacrylamide gel electrophoresis , biology , pilus , virulence , chemistry , escherichia coli , biochemistry , bacteria , enzyme , gene , genetics , anatomy
The fimbriae (E 4 ) of a virulent strain of Salmonella typhimurium were purified by ion exchange chromatography in an FPLC system. They had a channelled appearance under transmission electron microscope and showed a major structural subunit of 17‐kDa on sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. The purified fimbriae were found to agglutinate guinea pig erythrocytes, but this effect was inhibited in presence of D‐mannose. Immune sera raised against the Mono‐Q purified fimbriae (E 4 ) showed cross‐reactivity with the type‐1 fimbriae (F 1 ) composed of 21‐kDa fimbrin subunit, purified by a different method from the same strain.