Open AccessGenetic relationship between mouse adenovirus‐2 (strain K87) and human adenovirus‐2
Author(s) -
Jacques Chantal,
D'Amours Benoit,
Hamelin Claude
Publication year - 1994
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1994.tb06606.x
Subject(s) - bglii , adenovirus genome , biology , ecori , hindiii , restriction enzyme , genetics , microbiology and biotechnology , genome , dna , gene , homology (biology) , adenoviridae , mastadenovirus , strain (injury) , restriction map , virology , recombinant dna , plasmid , anatomy
The DNA of mouse adenovirus strain K87 (MAd‐2) was cloned and mapped with restriction endonucleases Bgl II, Cla I, Eco RI, Hin dII and Sph I. Large differences were found the MAd‐2 and MAd‐1 (strain FL) DNA molecules in terms of number and location of restriction sites. The MAd‐2 genome also appeared as larger size than in the MAd‐1 genome (34.72 kb vs. 30.14 kb). Our results confirm the existence of two distinct adenovirus species in the mouse. Hybridization experiments, on the other hand, indicate that both MAd‐1 and MAd‐2 are genetically related to human adenovirus type 2 (HAd‐2). Overlapping regions of DNA homology are located in genes coding for HAd‐2 structural components which could explain serological relationships observed between the human and the murine adenoviruses.