Open AccessDNA probes for identification of the ectomycorrhizal fungus Tuber magnatum Pico
Author(s) -
Lanfranco Luisa,
Wyss Peter,
Marzachí Cristina,
Bonfante Paola
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06581.x
Subject(s) - rapd , biology , primer (cosmetics) , intraspecific competition , genomic dna , interspecific competition , dna , polymerase chain reaction , genetics , dna profiling , botany , gene , zoology , population , genetic diversity , chemistry , demography , organic chemistry , sociology
The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber . RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), consistently appearing when genomic DNA was amplified with an aspecific primer (OPA‐18), was found to be a good marker for Tuber magnatum , and was used as a probe in Southern hybridization experiments. The specificity of the results suggests that this probe may be useful in developing specific primers for PCR amplifications.