Open AccessMultiple oral inoculations with Cryptosporidium parvum as a means of immunization for production of monoclonal antibodies
Author(s) -
Tilley Michael,
Eggleston Mark T.,
Upton Steve J.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06520.x
Subject(s) - monoclonal antibody , cryptosporidium parvum , antigen , immunization , inoculation , antibody , cryptosporidium , microbiology and biotechnology , biology , virology , immune system , immunology , feces
Oral immunization of suckling mice with Cryptosporidium parvum results in a humoral response to a limited set of antigens. Six‐day‐old BALB/c mice were each inoculated orally with 1 × 10 6 viable oocysts and subsequently administered oral inoculations of 2 × 10 6 viable oocysts at 30 and 60 days following the primary infection. After 45 days, mice were boosted with 1 × 10 6 oocysts orally, plus soluble extracts equivalent to 2 × 10 6 and 1 × 10 6 oocysts given intravenously and intraperitoneally, respectively. Four days later, splenic lymphocytes were fused to Ag8 myeloma cells. Using this method, we have been able to select for monoclonal antibodies that predominately recognize sporozoite surface and apical complex antigens.