Open AccessA DNA module encoding bph genes for the degradation of polychlorinated biphenyls (PCBs)
Author(s) -
Dowling David N.,
Pipke Rüdiger,
Dwyer Daryl F.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06506.x
Subject(s) - pseudomonas putida , operon , bacteria , polychlorinated biphenyl , strain (injury) , gene , genetically engineered , plasmid , chemistry , pseudomonas , biphenyl , dna , microbiology and biotechnology , microcosm , genetically modified organism , biology , escherichia coli , biochemistry , genetics , environmental chemistry , organic chemistry , anatomy
In this report we describe the development and construction of a DNA module which encodes bph genes for the metabolism of PCBs and which is capable of stable integration into the chromosome of Gram negative bacteria. Introduction of the bph ‐module into Pseudomonas putida KT2442, Pseudomonas sp. strain B13 and its genetically engineered derivative B13FR1 expanded the biodegradative ability of these strains to include biphenyl and 4‐chlorobiphenyl. The bph operon was stably inherited under laboratory conditions. Behavior of the genetically engineered strains was evaluated under simulated natural habitat conditions in lake sediment microcosms with respect to survival and removal of 4‐chlorobiphenyl. The genetically engineered strains persisted under these conditions and were effective in degrading 4‐chlorobiphenyl over a five day incubation period.