Open AccessEvaluation of macrolide and lincosamide antibiotics for plasmid maintenance in low pH Clostridium acetobutylicum ATCC 824 fermentations
Author(s) -
Mermelstein Lee D.,
Papoutsakis Eleftherios T.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06490.x
Subject(s) - tylosin , microbiology and biotechnology , lincomycin , spiramycin , oleandomycin , clostridium acetobutylicum , plasmid , chemistry , biology , antibiotics , erythromycin , biochemistry , butanol , ethanol , dna
Clarithromycin, midecamycin and tylosin effectively provided selective pressure for maintenance of a macrolide, lincosamide and streptogramin B resistance (MLS r ) plasmid in Clostridium acetobutylicum ATCC 824 at pH values as low as 4.5, whereas erythromycin, oleandomycin, spiramycin, lincomycin and clindamycin did not. Furthermore, clarithromycin, midecamycin and tylosin were not degraded by late stationary‐phase ATCC 824 fermentation fluids. Solvent formation, but not acid formation. was inhibited by tylosin but not by clarithromycin in an MLS r plasmid containing strain. Clarithromycin should therefore be useful for the maintenance of selective pressure for MLS r plasmids in low pH recombinant C. acetobutylicum ATCC 824 fermentations.