Open AccessUse of RAPD‐PCR to isolate a species specific DNA probe for Phytophthora cinnamomi
Author(s) -
Dobrowolski Mark P.,
O'Brien Philip A.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06485.x
Subject(s) - phytophthora cinnamomi , rapd , biology , dna , microbiology and biotechnology , agarose gel electrophoresis , polymerase chain reaction , southern blot , agarose , genetics , phytophthora , botany , gene , population , demography , sociology , genetic diversity
The products of RAPD‐PCR amplification of Phytophthora cinnamomi DNA were separated by electrophoresis in agarose. Parallel Southern blots of the gels were hybridized with nick translated DNA from different species of Phytophthora . Fragments that hybridized specifically to P. cinnamomi DNA were identified. These fragments were purified and cloned into pUC18. Their specificity for P. cinnamomi was confirmed.