Open AccessSynthesis of the Rhodopseudomonas viridis holo‐cytochrome c 2 in Paracoccus denitrificans
Author(s) -
Gerhus Ernst,
Grisshammer Reinhard,
Michel Hartmut,
Ludwig Bernd,
Turba Andreas
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06483.x
Subject(s) - paracoccus denitrificans , cytochrome , periplasmic space , biochemistry , biology , heme , mutagenesis , cytochrome p450 reductase , rhodopseudomonas palustris , cytochrome c , coenzyme q – cytochrome c reductase , mutant , gene , bacteria , genetics , enzyme , escherichia coli , mitochondrion
The gene encoding the Rhodopseudomonas viridis cytochrome c 2 (cycA) has been introduced on a broad host range vector into Paracoccus denitrificans , leading to high‐level expression of the holo‐cytochrome with the heme moiety covalently attached to the apoprotein. The cytochrome was demonstrated to reside in the periplasmic space of the host cell. In contrast to R. viridis , aerobic rather than anaerobic growth conditions led to higher production levels of the holo‐cytochrome in P. denitrificans . This heterologous expression system provides a suitable genetic background for the functional expression and mutagenesis of polypeptides involved in bacterial photosynthesis, offering the possibility of detailed structural and functional investigation.