Open AccessArabinan degrading enzymes from Aspergillus nidulans : Induction and purification
Author(s) -
Ramón Daniel,
Veen Peter v.d.,
Visser Jaap
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06481.x
Subject(s) - aspergillus nidulans , aspergillus niger , enzyme , biochemistry , aspergillus , chemistry , biology , microbiology and biotechnology , gene , mutant
The presence in Aspergillus nidulans of two enzymes related to the Aspergillus niger endo‐arabinase and α‐l‐arabinofuranosidase B has been established using antibodies against the purified A. niger enzymes. Moreover, the absence of an equivalent in A. nidulans to the α‐l‐arabinofuranosidase A of A. niger has been confirmed both at the protein and at the DNA level. Both A. nidulans arabinases have been purified and physico‐chemically and kinetically characterized. They have a much higher temperature optimum than the corresponding A. niger enzymes. The pattern of induction has been studied on media containing different carbon sources showing an important role of l‐arabitol in the induction of these enzymes.