Open AccessCloning, nucleotide sequence and expression of a ß‐lactamase gene from Streptomyces lavendulae
Author(s) -
Sendouda Atsuhito,
Urabe Hiroaki,
Ogawara Hiroshi
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06473.x
Subject(s) - nucleic acid sequence , microbiology and biotechnology , open reading frame , biology , nucleotide , gene , peptide sequence , molecular cloning , streptomyces , dna , restriction enzyme , genetics , biochemistry , bacteria
A hybridized DNA fragment was cloned as a 7.6‐kb fragment from Streptomyces lavendulae KCCS0263 using a 1.9‐kb Sac I‐ Xba I DNA fragment from S. cellulosae as a probe. The latter fragment encoded a ß‐lactamase which can bind blue dextran. The hybridized region was reduced to a 2.8‐kb Kpn I‐ Bcl I fragment and the nucleotide sequence was determined. The nucleotide sequence indicated one open reading frame whose amino acid sequence is very similar to that of the ß‐lactamase from S. cellulosae . The gene produced ß‐lactamase enzyme at a low but significant amount.