Open AccessPathological activities of Yersinia ruckeri , the Enteric Redmouth (ERM) bacterium
Author(s) -
Romalde Jesús L.,
Toranzo Alicia E.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06465.x
Subject(s) - microbiology and biotechnology , yersinia ruckeri , biology , bacteria , phospholipase a1 , trout , serotype , phospholipase , rainbow trout , enzyme , biochemistry , fish <actinopterygii> , fishery , genetics
The adherence and invasive capacities as well as the pathobiological activities exhibited by Yersinia ruckeri were examined. Although adhesive ability was dependent on the cell‐line employed, all the strains showed moderate adhesion and invasiveness in the salmon cell‐line CHSE‐214. With regard to the extracellular products (ECP) all of them were strongly toxic for fish with LD 50 ranging from 2 to 9.12 μg protein per g fish. In addition, all the ECP samples showed caseinase, gelatinase, amylase, lipase and phospholipase activities, hydrolysed esculin and displayed hemolytic activities for trout, salmon, sheep and human erythrocytes. Heat treatment (100°C for 10 min) caused the loss of all these biological activities except the hydrolysis of gelatin. On the other hand, SDS‐PAGE analysis of the LPS and protein components of the ECP revealed variations among strains depending on the serotype. The lack of lethal effects of the LPS present in the ECP was also demonstrated.