Open AccessMolecular subtyping within a single Salmonella typhimurium phage type, DT204c, with a PCR‐generated probe for IS 200
Author(s) -
Baquar Namoos,
Threlfall Eric John,
Rowe Bernard,
Stanley John
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06451.x
Subject(s) - subtyping , salmonella , typing , biology , phage typing , genotype , genetics , microbiology and biotechnology , polymerase chain reaction , dna , gene , bacteria , computer science , programming language
We report primers and conditions for the generation by PCR of a probe for the DNA insertion element IS 200 . This probe was shown to be suitable for genotypic subtyping within a single phage type of Salmonella typhimurium . A collection of isolates of DT204c, a phage type implicated in the spread of multiple drug resistance in bovine animals and to man, was analyzed. Three IS 200 profiles, corresponding to related chromosomal genotypes were characterized in DT204c. Molecular discrimination within a single phage type of S. typhimurium has general significance for genotypic typing, and for the definition of epidemiological clonality in Salmonella .