Open AccessPhylogenetic analysis of Bacillus sphaericus and development of an oligonucleotide probe specific for mosquito‐pathogenic strains
Author(s) -
Muro Marilena Aquino,
Priest Fergus G.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06449.x
Subject(s) - bacillus sphaericus , biology , microbiology and biotechnology , 16s ribosomal rna , gene , escherichia coli , phylogenetic tree , oligonucleotide , dna , genetics , bacteria , bacillales , bacillus subtilis
The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homologoy groups and one strain of Bacillus fusiformis have been determined. An oligonucleotide probe based on the area approximately between position 186 and 198 ( Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology group IIA which contains all known mosquito pathogens and not to any of ten other Bacillus species. It can be used to identify potential mosquito‐pathogenic strains of B. sphaericus in screening programmes.