Open AccessMolecular cloning and nucleotide sequence analysis of the maltose‐inducible porin gene of Aeromonas salmonicida
Author(s) -
Dodsworth S.J.,
Bennett A.J.,
Coleman G.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06447.x
Subject(s) - aeromonas salmonicida , biology , nucleic acid sequence , microbiology and biotechnology , peptide sequence , start codon , gene , open reading frame , molecular cloning , amino acid , restriction map , genetics , biochemistry , nucleotide , bacteria
The gene for the Aeromonas salmonicida maltose‐inducible porin (maltoporin) was cloned into phagemid pTZ18R in two restriction fragments, 0.6‐kb Pst I/ Kpn I and 1.7‐kb Sph I, of genomic DNA and their nucleotide sequences were determined. Open reading frames of 1329 and 1335 bp translated into sequences of 443 and 445 amino acids, with a 23 or 25 amino acid signal sequence and a 420 amino acid mature protein of molecular mass 46424 Da. Putative ribosome binding sites, AGGA and GGGAA, occurred 9 bp upstream of two possible ATG initiation codons. The A. salmonicida gene product showed a high degree of similarity with Escherichia coli LamB, and codon usage was very similar to that of another A. salmonicida outer membrane protein but markedly different from those of extracellular proteins.