Open AccessDetermination of genome size and restriction pattern polymorphism of Rickettsia prowazekii and Rickettsia typhi by pulsed field gel electrophoresis
Author(s) -
Eremeeva M.E.,
Roux V.,
Raoult D.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06431.x
Subject(s) - rickettsia prowazekii , rickettsia typhi , biology , pulsed field gel electrophoresis , restriction fragment length polymorphism , genome , genome size , genetics , rickettsiaceae , restriction fragment , restriction enzyme , dna , microbiology and biotechnology , gel electrophoresis , polymerase chain reaction , rickettsiales , rickettsia , genotype , gene , virus
Pulsed field gel electrophoresis (PFGE) of Sma I, Mlu I and Sal I digested DNA was used to estimate genome size and perform restriction fragment length polymorphism analysis for Rickettsia prowazekii and Rickettsia typhi . We concluded that the genome of R. prowazekii and R. typhi consisted of a single chromosomal DNA. The total length of DNA of R. prowazekii was 1,106±54 kb and of R. typhi was 1,133±44kb. It was possibleto differentiate two strains of R. prowazekii , Breinl and EVir, by PFGE analysis after Sal I digestion. Restriction fragment length polymorphism analysis did not reveal intraspecies differences between three human isolates and one Xenopsilla cheopis isolate of R. typhi .