Open AccessInvestigation of the role of the cydD gene product in production of a functional cytochrome d oxidase in Escherichia coli
Author(s) -
Bebbington Keren J.,
Williams Huw D.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06417.x
Subject(s) - mutant , escherichia coli , cytochrome , biochemistry , cytochrome c oxidase , biology , cytochrome b , respiratory chain , oxidase test , protein subunit , gene product , microbiology and biotechnology , gene , chemistry , gene expression , enzyme , mitochondrial dna
The cydD gene is needed for the formation of a functional cytochrome d oxidase in the aerobic respiratory chain of Escherichia coli . In this paper we demonstrate that transcription from a cydA‐lacZ gene fusion is not significantly affected in a cydD mutant. This, together with the finding that subunit I of cytochrome d is present in cytoplasmic membranes of a cydD mutant, rules out a role for CydD in the regulation of cytochrome d expression or the assembly of its polypeptides into the membrane. The activity of the haem d ‐containing catalase HP II was found to be similar in a cydD mutant and the wild‐type. Therefore, if CydD has a role in haem d biosynthesis it must be in a unique step only associated with the synthesis of the haem d prosthetic group of cytochrome d .