Open AccessInvestigation of the Pseudomonas aeruginosa ampR gene and its role at the chromosomal ampC β‐lactamase promoter
Author(s) -
Lodge Julia,
Busby Stephen,
Piddock Laura
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06404.x
Subject(s) - pseudomonas aeruginosa , gene , biology , promoter , genetics , pseudomonadales , microbiology and biotechnology , bacteria , gene expression
The complete sequence of the Pseudomonas aeruginosa β‐lactamase regulator gene ampR has been determined. The amino acid sequence encoded by this gene is compared with other members of the LysR family of transcription factors. The P. aeruginosa ampC promoter was subcloned and gel mobility shift assays were used to demonstrate specific binding of AmpR to the ampC promoter region.