Open AccessIsolation and characterization of Escherichia coli strains producing microcins of B and C types
Author(s) -
Khmel Inessa A.,
Bondarenko Victor M.,
Manokhina Irina M.,
Basyuk Eugenia I.,
Metlitskaya Anastasia Z.,
Lipasova Valentina A.,
Romanova Yulia M.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06397.x
Subject(s) - escherichia coli , plasmid , microbiology and biotechnology , biology , bacteria , enterobacteriaceae , bacteriocin , isolation (microbiology) , gram negative bacteria , gene , genetics
Abstract A screening of 11956 enterobacteria isolates resulted in selection of seven active microcin‐producing strains. The microcins were shown to be peptides or their derivatives with a rather broad spectrum of activity, mainly against Gram‐negative bacteria. According to cross‐immunity criteria, the microcins studied belonged to two of the previously suggested types, B (five strains) and C (two strains). Those of type B could be further classified into two subtypes on the account of differences in the spectrum of antibacterial activity. In five cases out of seven the microcin‐producing ability has been attributed to plasmids that the strains harboured. The effect of microcins on sensitive cells was shown to depend on ompR and ompF gene products.