Open AccessHeterologous gene expression in Campylobacter coli : The use of bacterial luciferase in a promoter probe vector
Author(s) -
Purdy Desmond,
Park Simon F.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06391.x
Subject(s) - biology , luciferase , plasmid , gene , bioreporter , photorhabdus luminescens , promoter , reporter gene , heterologous expression , expression vector , gene expression , campylobacter , vector (molecular biology) , heterologous , microbiology and biotechnology , genetics , bacteria , recombinant dna , transfection
A novel promoter probe plasmid (pSP73) was constructed to allow the analysis of environmentally regulated gene expression in Campylobacter . The vector utilizes the luxAB genes from Xenorhabdus luminescens , which encode a thermostable luciferase, as reporters of gene expression. The utility of this reporter system was demonstrated by placing the expression of luxAB under the transcriptional control of the flaA gene promoter.