Open AccessRegulation of ribosomal RNA transcription by growth rate of the hyperthermophilic Archaeon, Pyrococcus furiosus *
Author(s) -
DiRuggiero Jocelyne,
Achenbach Laurie A.,
Brown Stephen H.,
Kelly Robert M.,
Robb Frank T.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06379.x
Subject(s) - pyrococcus furiosus , hyperthermophile , ribosomal rna , 16s ribosomal rna , biology , gene , 23s ribosomal rna , transcription (linguistics) , archaea , genetics , rna , microbiology and biotechnology , ribosome , linguistics , philosophy
We have studied the single rRNA gene cluster from the Archaeon, Pyrococcus furiosus . This isolate grows optimally at 100°C and is thus a hyperthermophile. In P. furiosus , transcription of 16S rRNA is subject to regulation over a 7.5‐fold range in response to a 20‐fold increase in growth rate. The single cluster encoding the 16S and 23S rRNA genes of P. furiosus was cloned and the 1.9 kb region upstream of the 16S rRNA gene was sequenced.