Open AccessIsolation and characterization of a subsurface bacterium that degrades aniline and methylanilines
Author(s) -
Konopka Allan
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06367.x
Subject(s) - aniline , catechol , bacteria , chemistry , substrate (aquarium) , amine gas treating , strain (injury) , pseudomonas , nuclear chemistry , stereochemistry , biochemistry , organic chemistry , biology , genetics , ecology , anatomy
An aerobic bacterium was isolated from a subsurface sediment in a medium containing 2‐methylaniline as sole C and N source. The isolate, Pseudomonas strain AK20, grew on aniline and all isomers of methylaniline. Aromatic amine metabolism was inducible, and cell extracts contained catechol‐2,3‐oxygenase which was also active against methylcatechols. Growth on aniline was rapid ( μ = 0.41 h −1 ), and aniline and acetate were simultaneously utilized in mixed substrate cultures. Growth on 2‐methylaniline was slower ( μ = 0.1−0.17 h −1 ) and 2‐methylaniline was not used simultaneously with other organic substrates unless it served as sole N source.