Open AccessPrevalence of a Vibrio cholerae 18‐kDa antigen in vibrios
Author(s) -
Sciortino Carmen V.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06360.x
Subject(s) - vibrio cholerae , antigen , antiserum , microbiology and biotechnology , bacterial outer membrane , monoclonal antibody , biology , serotype , cholera , antibody , bacteria , vibrionaceae , western blot , virology , escherichia coli , immunology , biochemistry , gene , genetics
An 18‐kDa protein that occurs in Vibrio cholerae has been described as an in vivo and low‐iron regulated outer membrane antigen. Monoclonal antibodies which recognized this antigen were protective as passive vaccines in the infant rabbit model of cholera disease. In this study, those monoclonal antibodies were used in three immunological assays for surveillance of various bacteria for the 18‐kDa antigen. ELISA, and Western blot assays gave variable results with bacteria or outer membrane preparations. The biodot assay was the most sensitive test, detecting the 18‐kDa antigen in 29 of 29 V. cholerae strains, independent of biotype or serotype. A few other Gram‐negative bacteria and V. parahaemolyicus reacted weakly with our antibodies and antiserum.