Open AccessInsertional mutagenesis to isolate acetate‐requiring mutants in Chlamydomonas reinhardtii
Author(s) -
Adam Myriam,
Lentz Karl E.,
Loppes Roland
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06332.x
Subject(s) - chlamydomonas reinhardtii , insertional mutagenesis , mutant , mutagenesis , chlamydomonas , plasmid , biology , genetics , wild type , gene , escherichia coli
An arg 7 mutant of the green alga Chlamydomonas reinhardtii was transformed with pARG7.8, a plasmid bearing the wild‐type ARG 7 gene. Out of 4100 arg + transformants selected on an arginine‐free medium supplemented with acetate, nine failed to grow on acetate‐free medium (ac − mutants). The results of the genetic and molecular analysis of several ac − mutants are in agreement with the hypothesis that they originated from insertion of the incoming plasmid into the nuclear genome. These mutants should constitute valuable tools for isolating the corresponding wild‐type genes after plasmid rescue into Escherichia coli .