Open AccessIdentification and sequence analysis of the replication region of the phage resistance plasmid pCI528 from Lactococcus lactis subsp. cremoris UC503
Author(s) -
Lucey Mary,
Daly Charles,
Fitzgerald Gerald
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06330.x
Subject(s) - plasmid , biology , lactococcus lactis , genetics , hindiii , microbiology and biotechnology , sequence analysis , dna , bacteria , lactic acid
The replication region of the phage resistance plasmid pCI528 from Lactococcus lactis subsp. cremoris UC503 was localised to within a 10‐kb Hin dIII restriction fragment. A 6.3‐kb Bgl II‐ Hin dIII subclone of this fragment, cloned into a replication probe vector, allowed replication in Lactococcus but not in Bacillus or Lactobacillus . Sequence analysis revealed an ORF of 1152 bp preceded by a putative ori region containing a 22‐bp sequence tandemly repeated three and three‐quarter times, a second smaller direct repeat and two inverted repeats. Extensive homology was observed with the well characterised replication region of the small cryptic plasmid pCI305 (Hayes, F., Vos, P., Fitzgerald, G.F., de Vos, W. and Daly, C. Plasmid 25, 16–26).