Open AccessTransformation of Aspergillus alliaceus using the Aspergillus niger niaD gene encoding nitrate reductase
Author(s) -
Furlaneto Marcia C.,
Peberdy John F.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06314.x
Subject(s) - aspergillus niger , transformation (genetics) , nitrate reductase , plasmid , mutant , biology , biochemistry , dna , nitrate , heterologous , enzyme , gene , chemistry , microbiology and biotechnology , ecology
A heterologous transformation system for Aspergillus alliaceus based on the Aspergillus niger nitrate reductase structural gene ( niaD ) has been developed. Two mutants of A. alliaceus (M3 and M17), each carrying an niaD mutation were isolated by screening UV‐irradiated cells for the inability to grow on nitrate as sole nitrogen source. Using plasmid pSTA 10, transformation frequencies of 4 and 200 per μg DNA respectively were obtained for these two strains. All the niaD + transformants tested were mitotically stable. Southern hybridisation analyses showed that the vector DNA sequences were present.