Open AccessA double‐stranded RNA mycovirus from the plant pathogenic fungus, Fusarium solani f. sp. robiniae
Author(s) -
Nogawa M.,
Shimosaka M.,
Kageyama T.,
Okazaki M.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06312.x
Subject(s) - mycovirus , rna silencing , biology , virus like particle , pathogenic fungus , fusarium solani , rna , fungus , microbiology and biotechnology , fusarium , rna polymerase , botany , rna interference , biochemistry , gene , recombinant dna
Two kinds of double‐stranded RNA (dsRNA), estimated to be 1.9 and 1.7 kb in size, were detected in the plant pathogenic fungus, Fusarium solani f. sp. robiniae . Isometric virus‐like particles (VLPs), 30 nm in diameter, were recovered from cell extracts as a discrete band when centrifuged through a CsCl density gradient. The dsRNA molecules extracted from VLP preparations were identical in electrophoretic mobility to the dsRNAs obtained directly from cells. SDS‐PAGE analysis of the VLPs revealed a single polypeptide of 38 kDa. The dsRNAs obtained directly from cells. SDS‐PAGE analysis of the asexual cycle).