Open AccessSequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate
Author(s) -
Malissard Martine,
Duez Colette,
Guinand Micheline,
Vacheron MarieJeanne,
Michel Georges,
Marty Nicole,
Joris Bernard,
Thamm Iris,
Ghuysen JeanMarie
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06302.x
Subject(s) - escherichia coli , lyase , pseudomonas aeruginosa , plasmid , bacteria , microbiology and biotechnology , pseudomonas , biology , pseudomonadaceae , gene , lytic cycle , peptide sequence , biochemistry , recombinant dna , enzyme , genetics , virus
The recombinant plasmid pAL‐A3 bears a (poly ManA) alginate lyase‐encoding gene that originated from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870–1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL‐A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265‐amino acid residue alginate lyase showed lytic activity on a Pseudomonas aeruginosa alginate isolated from a cystic fibrosis patient. Unexpectedly, the alginate lyase thus characterized differed from that isolated from the culture medium of the bacterium ATCC 433367 (Romeo and Preston, Biochemistry (1986) 25, 8385–8391).