Open AccessThe operon for the Fe‐hydrogenase in Desulfovibrio vulgaris (Hildenborough): Mapping of the transcript and regulation of expression
Author(s) -
Berg Willy A.M.,
Stokkermans Jack P.W.G.,
Dongen Walter M.A.M.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06299.x
Subject(s) - operon , hydrogenase , desulfovibrio vulgaris , gene , biology , transcriptional regulation , transcription (linguistics) , messenger rna , lac operon , regulation of gene expression , genetics , gene expression , microbiology and biotechnology , bacteria , escherichia coli , linguistics , philosophy
The genes for the subunits of the Fe‐only hydrogenase from Desulfovibrio vulgaris are transcribed as a 1.9 kb mRNA; the operon contains no other genes besides those encoding the two subunits. The transcriptional start site of the operon was mapped. Determination of hydrogenase activity and hydrogenase mRNA levels indicates a growth‐phase dependent regulation of hydrogenase expression at transcriptional level. However, it has not yet been possible to localize the sequences required for regulation and expression of the genes.