Open AccessDifferentiation between isolates of Helicobacter pylori by PCR‐RFLP analysis of urease A and B genes and comparison with ribosomal RNA gene patterns
Author(s) -
Lopez Conchita Romero,
Owen Robert J.,
Desai Meeta
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06292.x
Subject(s) - haeiii , biology , restriction fragment length polymorphism , urease , helicobacter pylori , gene , ribosomal rna , genetics , polymerase chain reaction , restriction enzyme , 16s ribosomal rna , microbiology and biotechnology , enzyme , biochemistry
Genetic diversity amongst 21 human gastric isolates of Helicobacter pylori was investigated by polymerase chain reaction amplification and Hae III digest (restriction fragment length polymorphism) analysis of an internal 2.4‐kb segment of the urease A and urease B genes. H. pylori from 11 independent individuals yielded nine distinct restriction fragment patterns but only one pattern was common to H. pylori from two individuals. By contrast, multiple isolate sets of H. pylori from two patients each had common urease gene patterns. Most strains with the same urease gene patterns were distinguishable in their ribosomal RNA gene patterns. The study demonstrated diversity amongst H. pylori and established that PCR analysis of urease genes provided a novel method of identifying isolates. The profiles were reproducible and convenient to obtain and analyse, and were almost as discriminatory as Hae III ribopatterns.